Antibacterial Activity and Sanitizing Efficacy of HealthySole's Shoe UV Device (Kill Rate). Eight Second Activation Time

Microchem Laboratory B. Richard, B.S.

Organisms Tested:

Clostridium difficile (85.3%) ATCC 43598 (Endospores) 

Staphylococcus aureus (99.98%) ATCC 33592 (MRSA) 

Streptococcus pyogenes A (99.994%) ATCC 19615 

Enterococcus faecalis (99.75%) ATCC 51575 (VRE)

Escherichia coli (99.87%) ATCC BAA-2469 (CRE)

Pseudomonas aeruginosa (99.2%) ATCC 15442 


The test microorganism is prepared, usually by growth in liquid culture medium.

●  The test culture may be supplemented with an artificial soil load, such as horse or fetal bovine

serum, for one-step cleaner/sanitizer claims.

●  Sterilized carriers are inoculated with a volume of the test culture. Inoculated slides are dried.

Only completely dried carriers are used in the test.

●  Test carriers are treated with the test device and incubated for the predetermined contact time.

●  Control carriers are allowed to sit for the predetermined contact time, multiple controls may be

used for extended contact times.

●  At the conclusion of the contact time, test and control carriers are chemically neutralized.

●  Dilutions of the neutralized test substance are evaluated using appropriate growth media to

determine the surviving microorganisms at the respective contact time.

●  The effect of the test substance is compared to the effect of the control substance in order to

determine microbial reductions.